林晓霞;鹿炎;李会丽;周苹;郭新红;印丽萍;

• 1:湖南大学生物学院植物功能基因组学与发育调控湖南省重点实验室
• 2:上海市出入境检验检疫局

摘要(Abstract)：

采集了中国6个省份的铁皮石斛种质资源共22份,通过叶绿体基因matK和rbcL进行克隆测序,同时结合已报道的近缘物种matK序列33个和rbcL序列30个进行系统进化分析及分子鉴定。序列结果显示:matK基因序列平均长度为1 708 bp,GC含量32%,保守位点768个,简约信息位点871个;rbcL基因序列平均长度1 332 bp,GC含量42.9%,保守位点802个,简约信息位点24个。遗传多样性分析表明:matK序列单倍型数量(H)32个,单倍型多态性水平(Hd) 0.842,核苷酸多态性(π) 0.270 16;rbcL序列H为10个,Hd为0.664,π为0.026 11;序列分析及遗传多样性显示matK序列的分析结果具有更大的遗传多样性。遗传聚类结果显示不同产地的铁皮石斛与已有报道的石斛属各聚一支,相同产地铁皮石斛的聚类结果也与其地理分布基本一致。matK序列作为石斛属系统进化关系及亲缘关系分析具有更高的遗传多样性;中国6个省份铁皮石斛种质资源在进化上也具有一定的地域性特征。

关键词(KeyWords)： 石斛属;铁皮石斛;叶绿体基因;系统进化树

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFF0210303);; 国家自然科学基金项目(31872866,31540064);; 湖南省重点研发计划项目(2019NK2081,2017SK2182)

作者(Author): 林晓霞;鹿炎;李会丽;周苹;郭新红;印丽萍;

Email:

DOI:

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