唐俊妮;史贤明;王红宁;周锐;

• 1:四川大学生命科学学院动物疾病防控生物工程研究中心
• 2:华中农业大学农业微生物国家重点实验室
• 3:上海交通大学食品科学与工程系陆伯勋食品安全研究中心

摘要(Abstract)：

细菌通过自身合成的水合多聚物粘附在固体表面,以固着的方式生长从而形成生物膜,细菌生物膜的形成涉及到几个明显的阶段,包括起始的附着、细胞与细胞之间的吸附与增殖、生物膜的成熟、及最后细菌的脱离等四个阶段,生物膜的形成增加了细菌对抗生素的抗性以及帮助细菌逃逸寄主的免疫攻击等,从而引起临床上持续性的慢性感染等各种问题;生物膜结构非常复杂,除了细菌分泌的各种胞外多糖,胞外蛋白质外,最新的研究表明,DNA也是生物膜的一个重要成分。针对近年来的最新文献报道分别对生物膜的形成、结构以及调控机制等进行综述。

关键词(KeyWords)： 细菌生物膜;形成;结构;调控机制

Abstract:

Keywords:

基金项目(Foundation): “十一五”国家科技支撑计划重大项目“食品安全关键技术”子课题(2006BAK02A19);; 中国博士后专项基金

作者(Author): 唐俊妮;史贤明;王红宁;周锐;

Email:

DOI:

参考文献(References)：

• [1]Cvitkovitch D G,Li YH,Ellen R P.Quorumsensing and biofilmfor-mation in streptococcal infections[J].J Clin Invest,2003,112(11):1626~1632.
• [2]Kong K F,Vuong C,Otto M.Staphylococcus quorumsensing in bio-film formation and infection[J].Int J Med Microbiol,2006,296(2-3):133~9.
• [3]Massey R C,Horsburgh MJ,Lina G,et al.The evolution and main-tenance of virulence inStaphylococcus aureus:a role for host-to-hosttransmission[J].Nature,2006,4(12):953~958.
• [4]Schaik W,Abee T.The role ofσB in the stress response of Gram-positive bacteria-targets for food preservation and safety[J].CurrentOpinion in Biotechnology,2005,16:18~224.
• [5]Donlan R M,Costerton J W.Biofilms:survival mechanisms of clini-cally relevant microorganisms[J].Clin Microbiol Rev,2002,15(2):167~193.
• [6]Otto M.Virulence factors of the coagulase-negative staphylococci[J].Front.Biosci,2004,9:841~863.
• [7]Hall-Stoodley L,Stoodley P.Biofilm formation and dispersal and thetransmission of human pathogens[J].Trends Microbiol,2005,13:7~10.
• [8]Vuong C,Kocianova S,Yao Y,et al.Increased colonization of in-dwelling medical devices by quorum-sensing mutants ofStaphylococ-cus epidermidisin vivo[J].J Infect Dis,2004,190:1498~1505.
• [9]Orndorff P E,Devapali A,Palestrant S,et al.Immunoglobulin-media-ted agglutination and biofilm formation byEscherichia coliK-12 re-quires the type 1 pilus fiber[J].Infect Immun,2004,72:1929~1938.
• [10]Yarwood J M,Bartels D J,Volper E M,et al.Quorum sensing inStaphylococcus aureusbiofilms[J].J Bacteriol,2004,186:1838~1850.
• [11]Cynthia B W,Tolker-Nielsen T,Ragas P C,et al.ExtracellularDNArequired for bacterial biofilm formation[J].Science,2002,295(22):1487.
• [12]Pasmore M,Costerton J W.Biofilms,bacterial signaling,and theirties to marine biology[J].J Ind Microbiol Biotechnol,2003,30(7):407~413.
• [13]Podbielski A,Kreikemeyer B.Cell density-dependent regulation:basic principles and effects on the virulence of Gram-positive cocci[J].Int J Infect Dis,2004,8(2):81~95.
• [14]Beloin C,Valle J,Latour-Lambert P,et al.Global impact of maturebiofilm lifestyle onEscherichiacoli K-12 gene expression[J].Mol Mi-crobiol,2004,51(3):659~674.
• [15]Beloin C,Ghigo J M.Finding gene-expression patterns in bacterialbiofilms[J].Trends in Microbiology,2005,13(1):16~19.
• [16]O’Toole G,Kaplan H B,Kolter R.Biofilm formation as microbialdevelopment[J].Annu Rev Microbiol,2000,54:49~79.
• [17]Girard V,Mourez M.Adhesion mediated by autotransporters ofGram-negative bacteria:Structural and functional features[J].Research in Microbiology,2006,157:407~416.