黄银久;宋宝安;金林红;胡德禹;杨松;李宁;吴守伟;

• 1:蚌埠医学院生物科学系
• 2:贵州大学精细化工研究开发中心"绿色农药与生物工程"教育部重点实验室

摘要(Abstract)：

为了探索抗肿瘤药物体外筛选中磺酰罗丹明B(SRB)法和四甲基偶氮唑盐(MTT)比色法实验结果的相互验证作用,作者以PC-3、BGC-823、Bcap-37细胞系为研究对象,利用SRB法和MTT比色法对供试化合物行体外抑制活性考察,并用SPSS12.0对两种筛选方法得到的实验结果进行差异性分析。结果表明,SRB法和MTT比色法实验结果相关性好,可以相互验证实验结果的准确性和可靠性。

关键词(KeyWords)： MTT比色法;SRB法;相关性

Abstract:

Keywords:

基金项目(Foundation): 国家973计划(2003CB114404);; 科技部重点项目计划(2005DF100660);; 安徽省教育厅自然科学研究项目(2006KJ124C)

作者(Author): 黄银久;宋宝安;金林红;胡德禹;杨松;李宁;吴守伟;

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参考文献(References)：

• [1]Mosmann T.Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays[J].Journal of Im-munological Methods,1983,65(1-2):55～63.
• [2]Hayon T,Dvilansky A,Shpilberg O,et al.Appraisal of the MTT-based assay as a useful tool for predicting drug chemosensitivity in leukemia[J].Leukemia and Lymphoma,2003,44(11):1957～1962.
• [3]Hjarnaa P J,Jonsson E,Latini S,et al.CHS828,a novel pyridyl cy-anoguanidine with potent antitumor activityin vitroandin vivo[J].Cancer Research,1999,59:5751～5757.
• [4]陈陵际,韩家娴,杨蔚怡,等.灵芝精粉和孢子粉混合物抑制肿瘤细胞生长的实验研究[J].癌症,2002,21(12):1341～1344.
• [5]谢晓东,李小民,蒋芹,等.改良MTT法原代癌细胞培养药物敏感性试验的实验研究[J].陕西肿瘤医学,2001,9(4):230～232.
• [6]Fujimoto S,Akao T,Itoh B,et al.In vitro sensitivity test of stomach cancer tissues by the use of metal grid method[J].Japanese Journal of Surgery,1979,9(4):285～294.
• [7]Skehan P,Storeng R,Scudiero D,et al.NewColorimetric cytotoxic-ity assay for anticancer-drug screening[J].J Natl Cancer Inst,1990,82(13):1107～1112.
• [8]Sgouras D,Duncan R.Methods for the evaluation of biocompatibilityof soluble synthetic polymers which have potential for biomedical use:1-Use of the tetrazolium-based colorimetric assay(MTT)as a prelim-inary screen for evaluation ofin vitrocytotoxicity[J].Journal of Mate-rials Science:Materials in Medicine,1990,1:61～68.
• [9]Carmichael J,Mitchell J B,DeGraff W G,et al.Chemosensitivity testing of human lung cancer cell lines using the MTT assay[J].British Journal of Cancer,1988,57(6):540～547.
• [10]Sargent J M,Taylor C G.Appraisal of the MTTassay as a rapid test of chemosensitivity in acute myeloid leukaemia[J].British Journal of Cancer,1989,60(2):206～210.
• [11]唐亚辉,马葵,韩居然,等.MTT药物敏感试验在指导胃癌及原发肝癌化疗中的作用[J].肿瘤防治研究,2001,28(1):37～38.
• [12]Rotter B A,Thompson B K,Clarkin S,et al.Rapid colorimetric bioassay for screening of fusarium mycotoxins[J].Natural Toxins,1993,1(5):303～307.
• [13]赵荔华,刘超美,吴秋业,等.沙利度胺衍生物的设计合成及其抗肿瘤活性研究[J].中国药物化学杂志,2008,18(1):16～22.
• [14]张兴忠,徐莉英,陶淑娟,等.β-榄香烯醇酯类化合物的合成及抗癌活性研究[J].中国药物化学杂志,2007,17(1):13～17.