SRB法和MTT法抗肿瘤药物筛选结果相关性研究Correlation between experimental results of MTT colorimetric assay and SRB assay in anti-tumor drug screening in vitro
黄银久;宋宝安;金林红;胡德禹;杨松;李宁;吴守伟;
摘要(Abstract):
为了探索抗肿瘤药物体外筛选中磺酰罗丹明B(SRB)法和四甲基偶氮唑盐(MTT)比色法实验结果的相互验证作用,作者以PC-3、BGC-823、Bcap-37细胞系为研究对象,利用SRB法和MTT比色法对供试化合物行体外抑制活性考察,并用SPSS12.0对两种筛选方法得到的实验结果进行差异性分析。结果表明,SRB法和MTT比色法实验结果相关性好,可以相互验证实验结果的准确性和可靠性。
关键词(KeyWords): MTT比色法;SRB法;相关性
基金项目(Foundation): 国家973计划(2003CB114404);; 科技部重点项目计划(2005DF100660);; 安徽省教育厅自然科学研究项目(2006KJ124C)
作者(Author): 黄银久;宋宝安;金林红;胡德禹;杨松;李宁;吴守伟;
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