聂简琪;孙杨;杨艳坤;刘秀霞;李业;白仲虎;

• 1:江南大学粮食发酵工艺与技术国家工程实验室
• 2:江南大学工业生物技术教育部重点实验室
• 3:江南大学生物工程学院

摘要(Abstract)：

针对目前固定床生物反应器结构复杂、易产生泡沫等缺点,研发结构简单且可无泡通气的新型固定床生物反应器,并对该反应器的混合时间、k_La、细胞分布均匀性和细胞捕获能力进行测定。并进一步利用该反应器实施低密度接种Vero细胞进行细胞培养并扩增猪伪狂犬病毒。试验结果表明,该新型固定床反应器的混合时间、k_La与传统STR反应器相当,细胞捕获能力良好且细胞在反应器中分布均匀。采用低密度接种时Vero细胞可在该反应器中进行良好的生长,接毒5 d后累计收获的病毒总颗粒数达到10.37 log_(10) TCID_(50),相当于2.3×10~5剂量疫苗。该研究为细胞源疫苗的生产提供了新的反应器与技术。

关键词(KeyWords)： 固定床生物反应器;Vero细胞;猪伪狂犬病毒;低密度接种;疫苗

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.21878124,31570034);; 111计划(No.111-2-06);; 泰山产业领军人才(No.tscy20160307)

作者(Author): 聂简琪;孙杨;杨艳坤;刘秀霞;李业;白仲虎;

Email:

DOI:

参考文献(References)：

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