魏迪洋;闫利明;李巍然;贾绮林;

• 1:北京大学口腔医学院口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室
• 2:清华大学结构生物学实验室教育部蛋白质科学重点实验室

摘要(Abstract)：

利用X射线衍射晶体学方法解析IRF6蛋白的三维结构,同时验证IRF6对干扰素-β的转录调控作用,为IRF6蛋白的结构生物学研究提供基础。研究利用高通量基因克隆、蛋白表达与纯化获得大量的IRF6 N端蛋白结构域可溶性表达。凝胶迁移试验(EMSA)结果显示,IRF6 N端结构域可与干扰素-β启动子核酸片段形成蛋白-核酸复合体,具有明确的相互作用。将孵育后的蛋白-核酸复合体进行悬滴气相扩散法晶体筛选与优化后获得了X射线衍射分辨率达2.68?的晶体,为进一步揭示IRF6蛋白三维结构提供了帮助。验证了IRF6具有结合干扰素-β启动子元件特定片段的N端结构域,可调控干扰素-β的转录与表达,并通过IRF6 N端结构域与干扰素-β启动子核酸片段共同孵育后获得高分辨率蛋白晶体,以解析IRF6蛋白三维结构。研究结果对IRF6相关疾病机制研究及干扰素相关免疫通路的调控机制有重要意义。

关键词(KeyWords)： 干扰素调节因子6;干扰素-β;蛋白纯化;蛋白结晶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年项目(3170040488)

作者(Author): 魏迪洋;闫利明;李巍然;贾绮林;

Email:

DOI:

参考文献(References)：

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